The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation.

@article{Hannoun2010TheCB,
  title={The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation.},
  author={Zara Hannoun and Judy Fletcher and Sebastian Greenhough and Claire N. Medine and Kiyui. Samuel and Ruchi Sharma and Alexandra K A Pryde and James Richard Black and James Alexander Ross and Ian Wilmut and John Peter Iredale and David C. Hay},
  journal={Cellular reprogramming},
  year={2010},
  volume={12 2},
  pages={
          133-40
        }
}
Human embryonic stem cells (hESCs) offer an inexhaustible supply of human somatic cell types through their ability to self-renew while retaining pluripotency. As such, hESC-derived cell types are important for applications ranging from in vitro modeling to therapeutic use. However, for their full potential to be realized, both the growth of the undifferentiated cells and their derivatives must be performed in defined culture conditions. Many research groups maintain hESCs using mouse embryonic… CONTINUE READING
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