The comet assay as biomarker of heavy metal genotoxicity in earthworms.

@article{Reinecke2004TheCA,
  title={The comet assay as biomarker of heavy metal genotoxicity in earthworms.},
  author={Sophi{\'e} A Reinecke and Adriaan J Reinecke},
  journal={Archives of environmental contamination and toxicology},
  year={2004},
  volume={46 2},
  pages={208-15}
}
The ubiquitous occurring earthworm species, Eisenia fetida, were exposed to nickel chloride to determine whether the heavy metal Ni caused DNA damage, as measured by the comet (single cell gel electrophoresis) assay. Primary cell cultures of earthworm coelomocytes were exposed in vitro and whole animals either in spiked artificial soil water or in spiked cattle manure substrates. Comets formed were scored using mean tail lengths as well as comparing percentages of damage in five different… CONTINUE READING