The coenzyme A-synthesizing protein complex and its proposed role in CoA biosynthesis in bakers' yeast.

@article{Bucovaz1997TheCA,
  title={The coenzyme A-synthesizing protein complex and its proposed role in CoA biosynthesis in bakers' yeast.},
  author={Edsel T. Bucovaz and Robert M. Macleod and J. Calvin Morrison and Walter D. Whybrew},
  journal={Biochimie},
  year={1997},
  volume={79 12},
  pages={787-98}
}
An improved procedure is described for the recovery and purification of the coenzyme A-synthesizing protein complex (CoA-SPC) of Saccharomyces cerevisiae (bakers' yeast). The molecular mass of the CoA-SPC, determined prior to and following its purification, is estimated by Sephacryl S-300 size exclusion chromatography to be between 375,000-400,000. Two previously unreported catalytic activities attributed to CoA-SPC have been identified. One of these is CoA-hydrolase activity which catalyzes… CONTINUE READING