The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes.

@article{Fotheringham1986TheCA,
  title={The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes.},
  author={Ian G. Fotheringham and Sallie Dacey and P. A. Taylor and Terry J. Smith and Michael Glenn Hunter and Matthew S. Finlay and Sandy Blackadder Primrose and David Myatt Parker and Richard Mark Edwards},
  journal={The Biochemical journal},
  year={1986},
  volume={234 3},
  pages={
          593-604
        }
}
In this paper we describe the cloning and sequence analysis of the tyrB and aspC genes from Escherichia coli K12, which encode the aromatic aminotransferase and aspartate aminotransferase respectively. The tyrB gene was isolated from a cosmid carrying the nearby dnaB gene, identified by its ability to complement a dnaB lesion. Deletion and linker insertion analysis located the tyrB gene to a 1.7-kilobase NruI-HindIII-digest fragment. Sequence analysis revealed a gene encoding a 43 000 Da… CONTINUE READING
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