The cloning and characterization of the bacteriophage D108 regulatory DNA-binding protein ner.

@article{Tolias1985TheCA,
  title={The cloning and characterization of the bacteriophage D108 regulatory DNA-binding protein ner.},
  author={Peter P. Tolias and Michael S. Dubow},
  journal={The EMBO journal},
  year={1985},
  volume={4 11},
  pages={3031-7}
}
From the transposable Mu-like bacteriophage D108 we have cloned the ner gene under the control of the lac UV5 promoter in the expression vector pOP95-15. The recombinant plasmid, pPT011, overproduced the 8-kd D108 ner protein (visualized by in vitro-coupled transcription-translation) and served as a substrate for DNA sequencing of the D108 ner gene. The ner protein of D108 was found to be 48% homologous to the Mu ner protein, though the DNA sequences that encode these proteins are quite… CONTINUE READING