The cloning, sequence analysis, and expression in E. coli of coat protein gene of grapevine fanleaf virus Gh.

@article{Guan1996TheCS,
  title={The cloning, sequence analysis, and expression in E. coli of coat protein gene of grapevine fanleaf virus Gh.},
  author={Hua Guan and Wendy X. Cai and Kequiang Mang},
  journal={Chinese journal of biotechnology},
  year={1996},
  volume={12 2},
  pages={73-9}
}
Fragments of 5' end and 3' end of the coat protein gene of a Chinese strain grapevine fanleaf virus (GFLV-Gh) were separately amplified through RT-PCR. The two products were cloned and ligated into a complete full-length coat protein gene via an inherent BgIII restriction site. The entire gene contains 1512 nucleotides which encode a 504 amino acids coat protein, molecular weight approximately 56 kDa. Comparison with GFLV F-13 shows 88.4% homology in nucleotides and 95.8% similarity in deduced… CONTINUE READING