The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein.

@article{Mayfield1988TheCE,
  title={The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein.},
  author={John E. Mayfield and Betsy J Bricker and Henry Maduka Godfrey and Renae M. Crosby and David J W Knight and Shirley M. Halling and Doris Balinsky and Louisa B. Tabatabai},
  journal={Gene},
  year={1988},
  volume={63 1},
  pages={1-9}
}
Brucella abortus is the causative agent for brucellosis in cattle and man. Development of a single diagnostic test for the differentiation of vaccinated from infected animals and the development of a nonviable 'subunit' vaccine are top priorities of the brucellosis research program in the United States. Preliminary evidence previously showed that a purified 31-kDa protein (thought to be localized at or near the bacterial cell surface) protects against experimental brucellosis in rodents. The… CONTINUE READING

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