The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease

  title={The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease},
  author={Hans J{\"u}rgen Hoffmann and A F Santos and Cristobalina Mayorga and Anna Nopp and Bernadette Eberlein and Marta Ferrer and Paul Rouzaire and D. G. Ebo and Vito Sabato and Mar{\'i}a L Sanz and Tatjana Pecaric-Petkovic and Sarita U. Patil and Oliver V. Hausmann and Wayne G. Shreffler and Peter Koro{\vs}ec and Edward F. Knol},
  pages={1393 - 1405}
The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes. Basophil activation test measures basophil response to allergen cross‐linking IgE on between 150 and 2000 basophil granulocytes in <0.1 ml fresh blood. Dichotomous activation is assessed as the fraction of reacting basophils. In addition to clinical history, skin prick… 

Basophil activation testing in diagnosis and monitoring of allergic disease – an overview

An overview of the practical and technical details as well as the utility of the basophil activation test (BAT) in diagnosis and management of allergic diseases is provided.

Basophil activation test: Mechanisms and considerations for use in clinical trials and clinical practice

The basophil activation test (BAT) is a functional assay that measures the degree of degranulation following stimulation with allergen or controls by flow cytometry. It correlates directly with

Basophil Activation as Marker of Clinically Relevant Allergy and Therapy Outcome

  • B. Eberlein
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    Frontiers in Immunology
  • 2020
For some years now the basophil activation test (BAT) using flow cytometry has emerged as a powerful tool and sensitive marker that can be used to detect clinically relevant allergies, provide

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The applications of the BAT to the clinical management of allergic patients and the increased understanding of the mechanisms of immune response to allergens as well as technological advancements made in recent years are covered.

Clinical use of basophil activation test in drug, food and hymenoptera venom allergies.

Current state of BAT testing is presented focusing on the clinical laboratory parameters and issue of this assay, with considerations on further developments and clinical evidences still to be achieved.

The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions

The current data regarding the application of the BAT in food allergy (FA) for cow’s milk, egg and peanut, being the most common causes of FA in children are summarized.

Road map for the clinical application of the basophil activation test in food allergy

  • A. SantosW. Shreffler
  • Medicine, Biology
    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
  • 2017
Broad clinical application of BAT demands further standardization of the laboratory procedure and of the flow cytometry data analyses, as well as clinical validation of BAT as a diagnostic test for multiple target allergens and confirmation of its feasibility and cost‐effectiveness in multiple settings.

Basophil activation test in food allergy: is it ready for real-time?

  • T. KeswaniS. Patil
  • Biology, Medicine
    Current opinion in allergy and clinical immunology
  • 2021
Basophil activation testing is an effective biomarker for diagnosis and monitoring of food allergy and may provide a useful tool for management of food allergies.

Basophil Activation Test in IgE-Mediated Food Allergy: Should We Follow the Flow?

The utility and usefulness of basophil-activation experiments need to be reevaluated thoroughly, in view of difficulties inherent to the correct preparation and storage of allergen extracts, optimizing and standardizing stimulation conditions, and also the potential of alternative diagnostics such as component resolved diagnosis that are becoming more readily accessible.

Basophil activation test: food challenge in a test tube or specialist research tool?

The basophil activation test (BAT) has emerged as a new diagnostic test for food allergy that has high specificity, which confers a high degree of certainty in confirming the diagnosis of food allergy and allows deferring the performance of OFC in patients with a positive BAT.



The basophil activation test in immediate-type drug allergy.

Use of CD63 expression as marker of in vitro basophil activation in identifying the culprit in insect venom allergy.

In difficult cases of hymenoptera allergy, where history, skin tests and determination of specific antibodies do not allow a clear decision regarding the relevant insect species for immunotherapy, the additional performance of cellular tests (CAST and BAT) may be helpful.

Diagnostic Tests Based on Human Basophils: More Potentials and Perspectives than Pitfalls

A comprehensive review of clinically validated studies on allergy to aeroallergens, insect venoms, latex, food allergens and drugs, as well as chronic urticaria shows clearly that even with different protocols, reproducible and meaningful results can be obtained.

Recombinant allergens promote expression of CD203c on basophils in sensitized individuals.

Flow cytometric quantitation of CD203c on blood basophils exposed to recombinant allergens is a useful approach to determine the allergic state in sensitized individuals and represents a basis for a sensitive novel allergy test.

Clinical Routine Utility of Basophil Activation Testing for Diagnosis of Hymenoptera-Allergic Patients with Emphasis on Individuals with Negative Venom-Specific IgE Antibodies

The routine use of this cellular test should facilitate prescription of venom immunotherapy in complex cases with inconclusive diagnostic results and allow the identification of severe Hymenoptera-allergic patients with negative specific IgE and skin tests.

Basophil Activation Test Using Recombinant Allergens: Highly Specific Diagnostic Method Complementing Routine Tests in Wasp Venom Allergy

BAT performed with the recombinant allergens Ves v 5, Ves v 3 and Ves v 1 provides an emerging highly specific in vitro method for the detection of wasp venom allergy, compared to the sIgE detection.

Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy.

The diagnostic value of basophil activation test in patients with an immediate hypersensitivity reaction to radiocontrast media.

Monitoring of basophil activation using CD63 and CCR3 in allergy to muscle relaxant drugs.

The flow cytometry protocol is a promising tool in allergy diagnosis since it is specific and complementary to specific IgE detection, and the CD63 protocol showed good specificity.