The clinical significance of thymidine kinase 1 measurement in serum of breast cancer patients using anti-TK1 antibody.

@article{He2000TheCS,
  title={The clinical significance of thymidine kinase 1 measurement in serum of breast cancer patients using anti-TK1 antibody.},
  author={Qimin He and L Zou and Paul J Zhang and Joanna Lui and Sven Skog and Tommy Fornander},
  journal={The International journal of biological markers},
  year={2000},
  volume={15 2},
  pages={139-46}
}
The activity of total thymidine kinase in serum (S-TK) has been used as a tumor maker for decades. To date such activity has been determined using [125]I-iodo-deoxyuridine as a substrate. The aim of this study was to develop a new, antibody-based technique for the measurement of cytoplasmic thymidine kinase (TK1) in serum. Both mono- and polyclonal antibodies against S-TK1 were used in dot blot assay. S-TK1 was characterized by SDS and IEF techniques. Sixty-five breast cancer patients were… CONTINUE READING