The chromosomal DNA of Streptomyces lividans 66 is linear

  title={The chromosomal DNA of Streptomyces lividans 66 is linear},
  author={Y. Lin and H. Kieser and D. Hopwood and C. Chen},
  journal={Molecular Microbiology},
  • Y. Lin, H. Kieser, +1 author C. Chen
  • Published 1993
  • Biology, Medicine
  • Molecular Microbiology
  • Two copies of a DNA sequence similar or identical to one end of the linear plasmid SLP2 were found on the Streptomyces lividans chromosome. Restriction mapping showed that these sequences represented free ends. Electrophoretic retardation and glass‐binding studies indicated that the telomeres carry covalently bound proteins. Moreover, the chromosome migrated as an 8Mb linear DNA in pulsed‐field gel electrophoresis. A similar finding with the chromosomes of six other Streptomyces species… CONTINUE READING
    211 Citations
    Isolation and cloning of Streptomyces terminal fragments
    • 5
    • Highly Influenced
    Genetic instability of the Streptomyces chromosome
    • 119
    Conjugative DNA transfer in Streptomyces by TraB: is one protein enough?
    • 20
    • PDF
    Instability of artificially circularized chromosomes of Streptomyces lividans
    • 36
    Streptomyces linear plasmids that contain a phage‐like, centrally located, replication origin
    • 50
    • Highly Influenced


    Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.
    • 360
    • PDF
    Linear plasmids of Borrelia burgdorferi have a telomeric structure and sequence similar to those of a eukaryotic virus.
    • 140
    • PDF
    Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid.
    • D. Shiffman, S. Cohen
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1992
    • 52
    • PDF
    A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome.
    • 189
    • PDF
    Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts.
    • 67
    • PDF
    Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent.
    • M. S. Ferdows, A. Barbour
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1989
    • 143