The chromosomal DNA of Streptomyces lividans 66 is linear

@article{Lin1993TheCD,
  title={The chromosomal DNA of Streptomyces lividans 66 is linear},
  author={Yi-Shing Lin and Helen M. Kieser and David. Hopwood and Carton W. Chen},
  journal={Molecular Microbiology},
  year={1993},
  volume={10}
}
Two copies of a DNA sequence similar or identical to one end of the linear plasmid SLP2 were found on the Streptomyces lividans chromosome. Restriction mapping showed that these sequences represented free ends. Electrophoretic retardation and glass‐binding studies indicated that the telomeres carry covalently bound proteins. Moreover, the chromosome migrated as an 8Mb linear DNA in pulsed‐field gel electrophoresis. A similar finding with the chromosomes of six other Streptomyces species… Expand
Physical map of the linear chromosome of Streptomyces griseus
The chromosomal DNA of Streptomyces griseus 2247 (a derivative of strain IFO3237) was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE).Expand
Replication of the linear chromosomal DNA from the centrally located oriC of Streptomyces ambofaciens revealed by PFGE gene dosage analysis.
TLDR
A pulsed-field gel electrophoresis time-course analysis revealed that sequences flanking oriC were overrepresented relative to the rest of the chromosomal DNA during rapid growth, indicating that this origin is active. Expand
The terminal proteins of linear Streptomyces chromosomes and plasmids: a novel class of replication priming proteins
TLDR
This study demonstrated that the TP was covalently bound to the 5′ ends as proposed previously and constructed and used to purify the TPs of the Strep‐tomyces coelicolor A3(2) chromosome. Expand
Isolation and cloning of Streptomyces terminal fragments
TLDR
Using a method for the visualisation of terminal DNA fragments in agarose gels, it was possible to see three fragments in S. rimosus and five fragments in avermitilis and to clone the 298 bp BamHI fragment carrying the left end of plasmid SLP2. Expand
Genetic instability of the Streptomyces chromosome
TLDR
The Streptomyces wild‐type chromosome is linear in all examples studied, but undergoes very large deletions spontaneously at rates higher than 0.1% of spores and RecA seems to be involved in the amplification mechanism and control of genetic instability. Expand
Identification of DNA amplifications near the center of the Streptomyces coelicolor M145 chromosome.
TLDR
Two amplifications near the center of the Streptomyces coelicolor M145 chromosome are discovered, both located in the same region and share at least 3.6 kb of DNA. Expand
Conjugative DNA transfer in Streptomyces by TraB: is one protein enough?
TLDR
Only a single plasmid-encoded protein, TraB, is sufficient to translocate a double-stranded DNA molecule into the recipient in Streptomyces matings, and it recognizes clt-like sequences distributed all over the chromosome. Expand
The 387 kb linear plasmid pPZG101 of Streptomyces rimosus and its interactions with the chromosome.
TLDR
The linear plasmid pPZG101 of Streptomyces rimosus R6 was restriction mapped with the enzymes AseI, BfrI, DraI and XbaI and it suggested that at least three of the integrated strains had retained free plasmids ends. Expand
Instability of artificially circularized chromosomes of Streptomyces lividans
TLDR
The results indicate that the telomeres and/or certain terminal sequences may be involved in the structural instability of Streptomyces chromosomes. Expand
Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697.
TLDR
The six rrn loci of S. ambofaciens were cloned as recombinant cosmids and located on the AseI-Dŕal physical map of the linear chromosomal DNA, and the transcriptional orientation was determined relative to the physical map and was shown to be divergent away from an oriC-like locus. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 61 REFERENCES
A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.
TLDR
An inverted terminal repetition of 614 bp was found along with the presence of further interrupted homologous sequences beyond this area up to 800 bp, which are the first inverted terminal repeat sequences found in microbial linear plasmids. Expand
The conjugative plasmid SLP2 of Streptomyces lividans is a 50 kb linear molecule
TLDR
The SLP2 plasmid, demonstrated genetically to exist In Streptomyces lividans by its ability to promote conjugation and to elicit‘pocks’on recipient (SLP2−) cultures, is physically detected and partially homologous to a newly discovered 650 kb linear plasmids in S. parvulus. Expand
Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.
TLDR
A linkage map of the S. lividans chromosome containing ten markers was derived from the results of matings using several different sex plasmids, and protoplast fusions, to study a range of Streptomyces plasmid ability to promote their own transfer and to mobilize chromosomal markers. Expand
Linear plasmids of Borrelia burgdorferi have a telomeric structure and sequence similar to those of a eukaryotic virus
TLDR
Findings suggest that the novel linear plasmids of Borrelia originated through a horizontal genetic transfer across kingdoms. Expand
Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid.
  • D. Shiffman, S. Cohen
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1992
TLDR
The reconstruction of a functional linear replicon, the 12-kilobase Streptomyces clavuligerus plasmid pSCL, from separate DNA fragments cloned in Escherichia coli on the pUC19 plasmids is reported, indicating the existence of a cryptic origin of circular replication within the linear plasmide. Expand
A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome
TLDR
PFGE analysis of the Streptomyces coelicolor A3(2) chromosome revealed that the two long opposite quadrants of the genetic map that are almost devoid of markers are indeed physically long rather than being hot spots for genetic exchange, and must therefore contain long stretches of DNA different in function from the remainder of the genome. Expand
Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts
  • X. Wu, K. Roy
  • Biology, Medicine
  • Journal of bacteriology
  • 1993
TLDR
The larger of the two RNA species was present at a high level during the early stage of growth in liquid medium, and then its apparent rate of transcription decreased and remained at a lower level through the later stages; the level of the smaller RNA species remained relatively constant through all stages of growth. Expand
Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent.
TLDR
Using pulsed-field gel electrophoresis, studies indicate that Borrelia spirochetes, perhaps uniquely among prokaryotic organisms, have linear chromosomes. Expand
Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent.
  • M. S. Ferdows, A. Barbour
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1989
TLDR
Using pulsed-field gel electrophoresis, studies indicate that Borrelia spirochetes, perhaps uniquely among prokaryotic organisms, have linear chromosomes. Expand
Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron.
TLDR
This work has confirmed that Streptomyces lividans DNA contains a modification which makes it susceptible to double-strand cleavage during electrophoresis in buffers contaminated with ferrous iron, and suspects that many reports of "poor" preparations of S. lividan plasmids may be due to the above effect. Expand
...
1
2
3
4
5
...