The chromatin environment shapes DNA replication origin organization and defines origin classes.

@article{Cayrou2015TheCE,
  title={The chromatin environment shapes DNA replication origin organization and defines origin classes.},
  author={Christelle Cayrou and B. Ballester and I. Peiffer and Romain Fenouil and P. Coulombe and J. Andrau and J. van Helden and M. Méchali},
  journal={Genome research},
  year={2015},
  volume={25 12},
  pages={
          1873-85
        }
}
To unveil the still-elusive nature of metazoan replication origins, we identified them genome-wide and at unprecedented high-resolution in mouse ES cells. This allowed initiation sites (IS) and initiation zones (IZ) to be differentiated. We then characterized their genetic signatures and organization and integrated these data with 43 chromatin marks and factors. Our results reveal that replication origins can be grouped into three main classes with distinct organization, chromatin environment… Expand
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TLDR
This study revealed that DNase hypersensitivity, which often correlates with early replication at large-scale chromatin domains, did not emerge as a strong local determinant of origin activity, and found that two distinct sets of chromatin modifications exhibited strong local associations with two discrete groups of replication origins. Expand
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A new class of disordered elements controls DNA replication through initiator self-assembly
TLDR
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A new class of disordered elements controls DNA replication through initiator self-assembly
TLDR
It is reported that the replication initiation machinery of D. melanogaster unexpectedly undergoes liquid-liquid phase separation (LLPS) upon binding DNA in vitro and it is found that ORC, Cdc6, and Cdt1 contain intrinsically disordered regions (IDRs) that drive LLPS and constitute a new class of phase separating elements. Expand
Delving into Eukaryotic Origins of Replication Using DNA Structural Features
TLDR
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TLDR
A model is proposed in which excessive MCMs are actively loaded in a genome-wide manner, irrespective of chromatin status, but only a fraction are passively fired in chromatin areas with an accessible open structure, such as regions upstream of TSSs of transcribed genes. Expand
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TLDR
This work examines pluripotent, primary, differentiating, and immortalized human cells, and demonstrates that a class of origins, termed core origins, is shared by different cell types and host ~80% of all DNA replication initiation events in any cell population. Expand
Differences in firing efficiency, chromatin and transcription underlie the developmental plasticity of the Arabidopsis originome
TLDR
The originome of an organism at the postembryo stage is provided that should allow us to study ORI biology in response to development, environment and mutations with a quantitative approach and the computational strategies developed here can be transferred to other eukaryotic systems. Expand
Arabidopsis DNA replication initiates in intergenic , AT-rich open 4 chromatin 5
26 The selection and firing of DNA replication origins play key roles in ensuring that eukaryotes accurately 27 replicate their genomes. This process is not well documented in plants due in largeExpand
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Bioinformatic analyses showing that the previously identified GC-rich sequence elements form origin G-rich repeated elements (OGREs) that are present in 67% to 90% of the DNA replication origins from Drosophila to human cells, respectively and initiation of DNA synthesis takes place precisely at 160 bp and 280 bp from the OGRE. Expand
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