The cholesterol dependence of activation and fast desensitization of the nicotinic acetylcholine receptor.

@article{Rankin1997TheCD,
  title={The cholesterol dependence of activation and fast desensitization of the nicotinic acetylcholine receptor.},
  author={Susannah Rankin and George H Addona and Marek A Kloczewiak and Birgitte Bugge and Keith W Miller},
  journal={Biophysical journal},
  year={1997},
  volume={73 5},
  pages={2446-55}
}
When nicotinic acetylcholine receptors are reconstituted into lipid bilayers lacking cholesterol, agonists no longer stimulate cation flux. The kinetics of this process are difficult to study because variations in vesicle morphology cause errors in flux measurements. We developed a new stopped-flow fluorescence assay to study activation independently of vesicle morphology. When receptors were rapidly mixed with agonist plus ethidium, the earliest fluorescence increase reported the fraction of… CONTINUE READING

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