The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron–sulfur cluster formation in ferredoxin

@article{Ye2004TheCN,
  title={The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron–sulfur cluster formation in ferredoxin},
  author={Hong Ye and Gulnara F. Garifullina and Salah E. Abdel-Ghany and Lihong Zhang and Elizabeth A H Pilon-Smits and Marinus Pilon},
  journal={Planta},
  year={2004},
  volume={220},
  pages={602-608}
}
Plastids are known to be able to synthesize their own iron–sulfur clusters, but the biochemical machinery responsible for this process is not known. In this study it is investigated whether CpNifS, the chloroplastic NifS-like cysteine desulfurase of Arabidopsis thaliana (L.) Heynh. is responsible for the release of sulfur from cysteine for the biogenesis of iron–sulfur (Fe–S) clusters in chloroplasts. Using an in vitro reconstitution assay it was found that purified CpNifS was sufficient for Fe… CONTINUE READING

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