The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b

@article{Porra2002TheCH,
  title={The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b},
  author={Robert J. Porra},
  journal={Photosynthesis Research},
  year={2002},
  volume={73},
  pages={149-156}
}
Over the last half century, the most frequently used assay for chlorophylls in higher plants and green algae, the Arnon assay [Arnon DI (1949) Plant Physiol 24: 1–15], employed simultaneous equations for determining the concentrations of chlorophylls a and b in aqueous 80% acetone extracts of chlorophyllous plant and algal materials. These equations, however, were developed using extinction coefficients for chlorophylls a and b derived from early inaccurate spectrophotometric data. Thus, Arnon… CONTINUE READING
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