The chemical synthesis of oligo- and poly-nucleotides by the phosphotriester approach

@article{Reese1978TheCS,
  title={The chemical synthesis of oligo- and poly-nucleotides by the phosphotriester approach},
  author={C. Reese},
  journal={Tetrahedron},
  year={1978},
  volume={34},
  pages={3143-3179}
}
  • C. Reese
  • Published 1978
  • Chemistry
  • Tetrahedron
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This review will summarize recent progress of liquid-phase oligonucleotide synthesis and also briefly introduce the challenge to develop a process that applies our original liquid-phase synthesisExpand
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This review selects some representative coupling agents used for internucleotide bond formation reactions in the phosphoramidite method, which is now the most widely employed method for the chemicalExpand
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These modifications, as exemplified by the synthesis of fully protected T12, T18, T24 and T38 in 80%, 77%, 70% and 50% yields respectively, should allow the ready synthesis of polynucleotides of even longer chain lengths by purely chemical methods. Expand
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.
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Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purifying of the final product containing 3'-5' phosphodiester linkages. Expand
Nucleoside 3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. IV. New method for removal of 2,2,2-trichloroethyl group and 31P NMR as a new tool for analysis of deblocking of internucleotide phosphate protecting groups.
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31P NMR spectroscopy has been proposed as a new tool for analysis of removal of internucleotide phosphate protecting groups-a crucial step in oligonucleotides synthesis via phosphotriester approach. Expand
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Approval is paid to 2,4,6-triisopropylbenzenesulphonyl 4-nitroimidazolide as a new condensing agent for the coupling of larger oligonucleotide blocks. Expand
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Abstract The synthesis of two fully-protected hexanucleotides (11a and 11b) via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotideExpand
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