The chemical structure of lysozyme substrates and their cleavage by the enzyme

@article{Sharon1967TheCS,
  title={The chemical structure of lysozyme substrates and their cleavage by the enzyme},
  author={Nathan Sharon},
  journal={Proceedings of the Royal Society of London. Series B. Biological Sciences},
  year={1967},
  volume={167},
  pages={402 - 415}
}
  • N. Sharon
  • Published 18 April 1967
  • Chemistry, Biology
  • Proceedings of the Royal Society of London. Series B. Biological Sciences
The isolation of a disaccharide, N-acetyl-β-D-glucosaminyl-(1 → 4) N-acetylmuramic acid (NAG-NAM) and a corresponding tetrasaccharide, from lysozyme digests of Micrococcus lysodeikticus cell walls, is described. These compounds have been used for the study of the enzymic activity of lysozyme. Digestion of the tetrasaccharide into disaccharide has been followed by (a) paper chromatography, (b) colorimetry, using the Morgan-Elson reaction, and (c) polarimetry. Lysozyme was found to catalyse… 

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