The chemical chaperone proline relieves the thermosensitivity of a dnaK deletion mutant at 42 degrees C.

@article{Chattopadhyay2004TheCC,
  title={The chemical chaperone proline relieves the thermosensitivity of a dnaK deletion mutant at 42 degrees C.},
  author={Madhab K. Chattopadhyay and Ren{\'e}e Kern and M Y Mistou and Abhaya M Dandekar and Sandra L. Uratsu and Gilbert Richarme},
  journal={Journal of bacteriology},
  year={2004},
  volume={186 23},
  pages={8149-52}
}
Since, like other osmolytes, proline can act as a protein stabilizer, we investigated the thermoprotectant properties of proline in vitro and in vivo. In vivo, elevated proline pools in Escherichia coli (obtained by altering the feedback inhibition by proline of gamma-glutamylkinase, the first enzyme of the proline biosynthesis pathway) restore the viability of a dnaK-deficient mutant at 42 degrees C, suggesting that proline can act as a thermoprotectant for E. coli cells. Furthermore, analysis… CONTINUE READING
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