The charged residues in the surface-exposed C-terminus of the Soybean mosaic virus coat protein are critical for cell-to-cell movement.

Abstract

The Soybean mosaic virus (SMV) coat protein (CP) is necessary for virion assembly and viral cell-to-cell and long-distance movements in plants. We previously showed that the C-terminal region of the SMV CP is required for CP self-interaction. In the present study, we generated SMV mutants containing CPs with single amino acid substitutions of the charged amino acids in the C-proximal region. Infectivity and cell-to-cell movement of the SMV mutants were examined in soybean plants. Through this genetic approach, we identified three charged amino acid residues (R245, H246, and D250) in the surface-exposed C-terminus of the SMV CP that are critical for virus cell-to-cell and long-distance movement. Our findings suggest that the identified charged amino acids in the surface-exposed C-terminus of SMV CP are critical for CP intersubunit interactions and thereby for cell-to-cell and long-distance movement and virion assembly.

DOI: 10.1016/j.virol.2013.07.033
0501002014201520162017
Citations per Year

68 Citations

Semantic Scholar estimates that this publication has 68 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Seo2013TheCR, title={The charged residues in the surface-exposed C-terminus of the Soybean mosaic virus coat protein are critical for cell-to-cell movement.}, author={Jang-Kyun Seo and Mi Sa Vo Phan and Sung-Hwan Kang and Hong-Soo Choi and Kook-Hyung Kim}, journal={Virology}, year={2013}, volume={446 1-2}, pages={95-101} }