The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.

@article{Kreft2015TheCO,
  title={The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.},
  author={Mateja Erdani Kreft and Ur{\vs}ka Dragin Jerman and Eva Lasi{\vc} and Neli Hevir-Kene and Tea Lanisnik Rizner and Luka Peternel and Katja Kristan},
  journal={European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences},
  year={2015},
  volume={69},
  pages={
          1-9
        }
}
In this study we have investigated the effects of different cell culture conditions on the Calu-3 epithelial cell model. Calu-3 cells were cultured in media A-MEM at the air-liquid (A-L) or liquid-liquid (L-L) interface for one or three wks (weeks). Different cryomethods were tested and the cell line was characterized using histochemistry, immunofluorescence, transmission and scanning electron microscopy, transepithelial resistance (TEER) measurements, permeability studies, and gene profiling… CONTINUE READING
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