The characterization for the binding of calcium and terbium to Euplotes octocarinatus centrin.

@article{Yaqin2009TheCF,
  title={The characterization for the binding of calcium and terbium to Euplotes octocarinatus centrin.},
  author={Zhao Yaqin and Feng Jiuying and Liang Aihua and Yang Binsheng},
  journal={Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy},
  year={2009},
  volume={71 5},
  pages={1756-61}
}
Centrin is a member of the EF-hand superfamily that plays critical role in the centrosome duplication and separation. In the present paper, we characterized properties of metal ions binding to Euplotes octocarinatus centrin (EoCen) by fluorescence spectra and circular dichroism (CD) spectra. Changes of fluorescence spectra and alpha-helix contents of EoCen proved that Tb(3+) and Ca(2+) induced great conformational changes of EoCen resulting in exposing hydrophobic surfaces. At pH 7.4, Ca(2… CONTINUE READING
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