The characteristics, capacity and receptor regulation of inositol uptake in 1321N1 astrocytoma cells.

@article{Batty1993TheCC,
  title={The characteristics, capacity and receptor regulation of inositol uptake in 1321N1 astrocytoma cells.},
  author={Ian H. Batty and Andrew Michie and M Fennel and C. Peter Downes},
  journal={The Biochemical journal},
  year={1993},
  volume={294 ( Pt 1)},
  pages={49-55}
}
The uptake of inositol into 1321N1 astrocytoma cells was studied by measurement of the accumulation of free [3H]inositol within the intracellular pool. Uptake occurs via a saturable transporter with apparent Km for inositol approximately 40 microM and Vmax approximately 180 pmol/min per mg of protein, which permits intracellular inositol concentrations to exceed those of the medium by a factor of approximately 500. At extracellular concentrations up to 500 microM, inositol uptake is highly… CONTINUE READING