The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro

@article{Prelich1987TheCR,
  title={The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro},
  author={Gregory Prelich and Matthew J. Kostura and Daniel Robert Marshak and Michael B. Mathews and Bruce W. Stillman},
  journal={Nature},
  year={1987},
  volume={326},
  pages={471-475}
}
Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr ∼36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin. 
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein
TLDR
It is shown that PCNA and the polyrnerase-δ auxiliary protein have similar electrophoretic behaviour and are both recognized by anti-PCNA human autoantibodies, and both proteins are functionally equivalent; they stimulate SV40 DNA replication in vitro and increase the processivity of calf thymus DNA polymerase- δ. Expand
Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells.
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This observation provides a direct link between a protein required for DNA replication and cell-cycle-regulated protein phosphorylation, thereby resetting this cycle. Expand
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  • E. Prosperi
  • Chemistry, Medicine
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  • 1997
TLDR
A role for PCNA in the cell cycle control is recognised on the basis of the interaction with cyclins, cyclin-dependent kinases and the cdk-inhibitor p21 waf1/cip1/sdi1 protein. Expand
The promoter of the proliferating cell nuclear antigen (PCNA) gene is active in serum-derived cells.
TLDR
Levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels doe not depend on the 5' flanking sequence of the PCNA gene. Expand
Identification of cellular components required for SV40 DNA replication in vitro.
TLDR
The results suggest a model for DNA synthesis which involves multiple stages prior to and during replicative DNA synthesis, and suggests that both polymerases alpha and delta are involved in this system. Expand
Proliferating Cell Nuclear Antigen : A New Marker of Proliferation in Cancer
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Assays of PCNA can complement DNA analysis for assessment of proliferative activity in malignant cells and tissues because of the potential for substantial variability between proliferating cell populations with respect to the proportions of cells in G0 or G1. Expand
Proliferating cell nuclear antigen is required for DNA excision repair
TLDR
The ability to visualize repair intermediates in the absence of PCNA facilitates dissection of the multiprotein reaction that leads to incision of damaged DNA in a major pathway of cellular defense against mutagens. Expand
Requirement for proliferating cell nuclear antigen expression during stages of the Chinese hamster ovary cell cycle.
TLDR
It is confirmed that the quantity of PCNA/cyclin protein in the cells increases severalfold in G-1 or early S phase but generally is invariant in S and G-2/M phases, which suggests that the observed synthesis is a prerequisite for initiation of DNA replication. Expand
Cellular factors required for papillomavirus DNA replication
TLDR
Interestingly, replication factor C and proliferating-cell nuclear antigen are more stringently required for DNA synthesis in the papillomavirus system than in the simian virus 40 in vitro system, indicating that there must be mechanistic differences between the DNA replication systems of papilloavirus and simianirus 40. Expand
Addition of calmodulin antagonists to NRK cells during G1 inhibits proliferating cell nuclear antigen expression.
TLDR
It can be suggested that calmodulin activates the release of a transcriptional block leading to an increase in the amount of PCNA during S phase, which is essential for the synthesis of the leading and lagging strands of DNA. Expand
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It is shown that PCNA and the polyrnerase-δ auxiliary protein have similar electrophoretic behaviour and are both recognized by anti-PCNA human autoantibodies, and both proteins are functionally equivalent; they stimulate SV40 DNA replication in vitro and increase the processivity of calf thymus DNA polymerase- δ. Expand
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