The cell culture medium — a functional extracellular compartment of suspension-cultured cells

  title={The cell culture medium — a functional extracellular compartment of suspension-cultured cells},
  author={Michael Wink},
  journal={Plant Cell, Tissue and Organ Culture},
The spent medium of suspension-cultured cells of Lupinus polyphyllus was analyzed by capillary GC and GC-MS and shown to contain ethanol (up to 160 mmol l-1), organic acids (lactate, benzoate, succinate, fumarate, malate), amino acids (main components: alanine, glycine, serine, aspartate, ornithine, glutamate), and quinolizidine alkaloids (lupanine and an uncharacterized malonylderivative). In addition, cells obviously secrete polysaccharides and enzymes (acid phosphatase, phosphodiesterase… CONTINUE READING


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