The catalytic domain of the germination-specific lytic transglycosylase SleB from Bacillus anthracis displays a unique active site topology.

@article{Jing2012TheCD,
  title={The catalytic domain of the germination-specific lytic transglycosylase SleB from Bacillus anthracis displays a unique active site topology.},
  author={Xing Jing and Howard Robinson and Jared D. Heffron and David L Popham and Florian David Schubot},
  journal={Proteins},
  year={2012},
  volume={80 10},
  pages={2469-75}
}
Bacillus anthracis produces metabolically inactive spores. Germination of these spores requires germination-specific lytic enzymes (GSLEs) that degrade the unique cortex peptidoglycan to permit resumption of metabolic activity and outgrowth. We report the first crystal structure of the catalytic domain of a GSLE, SleB. The structure revealed a transglycosylase fold with unique active site topology and permitted identification of the catalytic glutamate residue. Moreover, the structure provided… CONTINUE READING