The catalytic activity of cytochrome P450cam towards styrene oxidation is increased by site-specific mutagenesis.

@article{Nickerson1997TheCA,
  title={The catalytic activity of cytochrome P450cam towards styrene oxidation is increased by site-specific mutagenesis.},
  author={Diane Nickerson and C F Harford-Cross and S R Fulcher and Luet Lok Wong},
  journal={FEBS letters},
  year={1997},
  volume={405 2},
  pages={153-6}
}
The styrene oxidation activity of cytochrome P450cam, has been greatly improved by rational protein engineering. Compared to the wild-type enzyme, the active-site mutants Y96A and Y96F bound styrene more tightly, consumed NADH more rapidly, and were more efficient at utilising reducing equivalents for product formation. Styrene oxide formation rates were enhanced 9-fold in the Y96A mutant relative to wild-type, and 25-fold in the Y96F mutant, thus demonstrating the effectiveness of active-site… CONTINUE READING