The carboxyproxyl-derived spin trap (CP-H) is an appropriate detector-compound for oxidative stress

  title={The carboxyproxyl-derived spin trap (CP-H) is an appropriate detector-compound for oxidative stress},
  author={S Adam and Hagen Loertzer and P. Fornara and H. J. Br{\"o}mme},
  journal={Urological Research},
Reperfusion of ischemic tissue disturbs the balance between reactive oxygen species (ROS) and the cellular antioxidative defense. This imbalance is known as oxidative stress. In this study the spin trap 3-carboxy-2,2,5,5-tetramethylpyrrolin-1-hydroxide (CP-H) with its ESR-detectable paramagnetic analogue 3-carboxy-2,2,5,5-tetramethylpyrrolin-1-oxyl (•CP) was analyzed in vitro and in vivo. In preliminary in vitro experiments we studied the interaction of CP-H with reactive compounds like… 

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A High Precision Method for Quantitative Measurements of Reactive Oxygen Species in Frozen Biopsies

The absolute spin concentration in wet biological samples such as biopsies, water solutions and cell cultures could be quantified with higher precision and accuracy than normally achievable using common techniques such as flat cells, tissue cells and various capillary tubes.

Molecular hydrogen in sports medicine: new therapeutic perspectives.

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    International journal of sports medicine
  • 2015
Hydrogen therapy may be an effective and specific innovative treatment for exercise-induced oxidative stress and sports injury, with potential for the improvement of exercise performance.

Usefulness of Thulium-Doped Fiber Laser and Diode Laser in Zero Ischemia Kidney Surgery—Comparative Study in Pig Model

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A new approach for extracellular spin trapping of nitroglycerin-induced superoxide radicals both in vitro and in vivo.

Quantification of superoxide radicals and peroxynitrite in vascular cells using oxidation of sterically hindered hydroxylamines and electron spin resonance.

Hydroxylamines TEMPONE-H and CP-H can be used as nontoxic compounds in ESR assay capable of quantifying the formation of superoxide radicals and peroxynitrite in suspensions of cells and in the whole blood with high sensitivity.

Spin trapping of superoxide radicals and peroxynitrite by 1-hydroxy-3-carboxy-pyrrolidine and 1-hydroxy-2,2,6, 6-tetramethyl-4-oxo-piperidine and the stability of corresponding nitroxyl radicals towards biological reductants.

CP-H is more suitable for spin trapping of superoxide radicals and peroxynitrite in biological systems than the TEMPONE-H.

Quantitative measurement of superoxide generation using the spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide.

DEPMPO is an efficient spin trap which enables specific and sensitive detection and quantitation of superoxide generation and adduct decay and was 40-fold more sensitive than cytochrome c.

Evidence for only a moderate lipid peroxidation during ischemia-reperfusion of rat kidney due to its high antioxidative capacity

It could be analytically and stoichiometrically shown that after IR there is only a moderate oxidative stress in kidneys of young rats; this is due to their high SoA-scavenging capacity compared with their SOA-generating ability.

Biology of free radical scavengers: an evaluation of ascorbate

  • R. C. RoseA. Bode
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    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1993
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Uric acid-iron ion complexes. A new aspect of the antioxidant functions of uric acid.

The present results provide a mechanistic explanation for the previous report that urate protects ascorbate from oxidation in human blood and demonstrate that another important 'antioxidant' property of uric acid is the ability to form stable co-ordination complexes with iron ions.

Xanthine oxidase: evidence against a causative role in renal reperfusion injury.

XO-derived superoxide anion radicals cannot be considered causative for LPO in the reperfusion interval of experimental ischemia-reperfusion model of acute renal failure in the rat.