The carboxyl terminus of coffee bean alpha-galactosidase is critical for enzyme activity.

@article{Maranville2000TheCT,
  title={The carboxyl terminus of coffee bean alpha-galactosidase is critical for enzyme activity.},
  author={E Maranville and Aiping Zhu},
  journal={Archives of biochemistry and biophysics},
  year={2000},
  volume={373 1},
  pages={225-30}
}
The role of the carboxyl (C)-terminal region of coffee bean alpha-galactosidase (alpha-GAL) has been studied by expressing C-terminal deletion mutants in the methylotrophic yeast strain Pichia pastoris. A previous study of human alpha-galactosidase determined that enzyme activity increased when up to 10 amino acid residues were deleted. Deleting 11 residues reduced activity, and deleting 12 residues abolished activity. In our studies, alpha-GAL activity is reduced when one or two amino acids… CONTINUE READING