The carboxyl-terminal region of staphylococcal enterotoxin type A is required for a fully active molecule.

@article{Hufnagle1991TheCR,
  title={The carboxyl-terminal region of staphylococcal enterotoxin type A is required for a fully active molecule.},
  author={W. O. Hufnagle and Mary T Tremaine and Marsha J. Betley},
  journal={Infection and immunity},
  year={1991},
  volume={59 6},
  pages={2126-34}
}
Staphylococcal enterotoxin type A (SEA) gene (sea+) mutations were constructed by exonuclease III digestion or cassette mutagenesis. Five different sea mutations that had 1, 3, 7, 39, and 65 codons deleted from the 3' end of sea+ were identified and confirmed by restriction enzyme and nucleotide sequence analyses. Each of these sea mutations was constructed in Escherichia coli and transferred to Staphylococcus aureus by using the plasmid vector pC194. Culture supernatants from the parent S… CONTINUE READING