The carbohydrate-protein binding region in keratan sulfate from bovine cornea. I. Isolation and partial characterization.

@article{Brekle1980TheCB,
  title={The carbohydrate-protein binding region in keratan sulfate from bovine cornea. I. Isolation and partial characterization.},
  author={A. Brekle and G. Mersmann},
  journal={Hoppe-Seyler's Zeitschrift fur physiologische Chemie},
  year={1980},
  volume={361 1},
  pages={
          31-9
        }
}
  • A. Brekle, G. Mersmann
  • Published 1980
  • Medicine, Biology
  • Hoppe-Seyler's Zeitschrift fur physiologische Chemie
We present a defined chemical method for the isolation of the oligosaccharide linking the protein to the disaccharide units of keratan sulfate from bovine corneas. 1) Hydrazinolysis removes the amino acids from the peptido keratan sulfate, and leads to deacetylation of the glucosamine residues but does not split off the sulfate groups. 2) NaB3H4 reduction selectively labels the reducing terminal glucosamine of the chain by converting it to [3H]glucosaminitol. 3) Nitrous acid deamination splits… Expand
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The carbohydrate-protein binding region in keratan sulfate from bovine cornea, structure of a major binding region oligosaccharide.
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