The capacity to retrieve escaped ER proteins extends to the trans-most cisterna of the Golgi stack

@article{Miesenbck1995TheCT,
  title={The capacity to retrieve escaped ER proteins extends to the trans-most cisterna of the Golgi stack},
  author={Gero Miesenb{\"o}ck and James E Rothman},
  journal={The Journal of Cell Biology},
  year={1995},
  volume={129},
  pages={309 - 319}
}
To explore how far into the Golgi stack the capacity to retrieve KDEL proteins extends, we have introduced an exogenous probe (the peptide YHPNSTCSEKDEL) into the TGN of living cells. For this purpose, a CHO cell line expressing a c-myc-tagged version of the transmembrane protein TGN38--which cycles between the TGN and the cell surface--was generated. The cells internalized peptides that were disulfide bonded to anti-myc antibodies and accumulated the peptide-antibody complexes in the TGN… CONTINUE READING
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Reduction of the disulfide bond of chromogranin B ( secrotogmain I ) in the tr ~ s - Golgi network causes its missorting to the constitutive secretory pathway

  • S. Munro
  • EMBO ( Eur . Mol . Biol Organ . ) J .
  • 1994

A new method for detecting endocymsed proteins

  • E. Chanat, U. Weiss, W. B. Huttner, S. A. Tooze
  • EMBO ( Fur . Mol . Biol . Organ . ) J .
  • 1993

Identification of a con - sansas motif for retention of transmembrane proteins in the endoplasmic reticulum

  • M. R. Jackson, T. Niisson, P. A. Peterson
  • EMBO ( Eur . Mol . Biol . Organ . ) J .
  • 1993

Polymorphisms in the coding and noncoding regions of routine Pgk - I alleles

  • C. Wraight
  • Biochem . Genet .
  • 1993

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