The calmodulin‐dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans.

  title={The calmodulin‐dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans.},
  author={C. Rasmussen and C. Garen and S. Brining and R. Kincaid and R. Means and A. Means},
  journal={The EMBO Journal},
The gene encoding the homologue of the catalytic subunit of the Ca2+/calmodulin‐regulated protein phosphatase 2B (calcineurin A) has been isolated from Aspergillus nidulans. This gene, cnaA+, is essential in this fungal system. Analysis of growth‐arrested cells following gene disruption by homologous recombination reveals that they are blocked early in the cell cycle. The cnaA+ gene encodes a 2.5 kb mRNA and the deduced protein sequence is highly homologous to the calcineurin A subunit of other… Expand
Calcium and Calmodulin Regulation of the Nuclear Division Cycle of Aspergillus Nidulans
The results further demonstrates a selective requirement of Ca 2+ and calmodulin for the initiation of mitosis and also identifies two critical mitotic kinases, p34 cdc2 and NIMA, as potential molecular targets for their action at this transition point of the division cycle. Expand
Regulation of Calcineurin Gene Expression in Schizosaccharomyces pombe
This study cloned the gene for the catalytic subunit of calcineurin, CnA, from the yeast Schizosaccharomyces pombe, and shows that induction of the ppb1 mRNA during nitrogen starvation is dependent on the ste11 gene product. Expand
Analysis of All Protein Phosphatase Genes in Aspergillus nidulans Identifies a New Mitotic Regulator, Fcp1
It is demonstrated that nimT and bimG have essential functions required for mitotic progression since their deletions led to classical G2- and M-phase arrest. Expand
Overexpression, purification and characterization of Dictyostelium calcineurin A.
The catalytic subunit of Ca2+/calmodulin-dependent protein phosphatase (calcineurin A) was overexpressed about 50-fold in Dictyostelium discoideum cells transformed with a vector containing the cDNA for D. discoideums under control of the actin-6 promoter, and the enzymatic activity of the isolated protein was characterized. Expand
Role of Ca++/calmodulin binding proteins in Aspergillus nidulans cell cycle regulation.
The goal of this review is to summarise the current knowledge concerning the targets of Ca++/calmodulin that are essential for cell cycle progression in lower eukaryotes. Emphasis is placed onExpand
Regulation of the Calmodulin-stimulated Protein Phosphatase, Calcineurin*
The demonstration that FK506 and CsA, when bound to their respective binding proteins, are specific inhibitors of calcineurin provided the tools needed to reveal its many other roles in the transduction of Ca signals. Expand
Calcineurin: form and function.
This review provides a comprehensive examination of the biological roles of calcineurin and reviews aspects related to its structure and catalytic mechanism. Expand
Cloning and sequence analysis of cnaA gene encoding the catalytic subunit of calcineurin from Aspergillus oryzae.
The deduced amino acid sequence was homologous to calcineurin A from Aspergillus nidulans, Neurospora crassa, human, Saccharomyces cerevisiae and Schizosaccharomyceces pombe, and was not viable in the presence of high concentrations of NaCl and alkaline pH 8.5. Expand
Calcineurin regulatory subunit is essential for virulence and mediates interactions with FKBP12–FK506 in Cryptococcus neoformans
A central role for calcineurin B in virulence and antifungal drug action in the human fungal pathogen C. neoformans is revealed and molecular modelling illustrates how this mutation perturbs drug interactions with the phosphatase target. Expand
The Gα subunit BCG1, the phospholipase C (BcPLC1) and the calcineurin phosphatase co‐ordinately regulate gene expression in the grey mould fungus Botrytis cinerea
Among the BCG1/calcineurin‐co‐regulated genes, a new gene cluster coding for a yet unknown polyketide secondary metabolite is found and it is shown for the first time in a phytopathogenic fungus that the phospholipase C (BcPLC1) is a component of theBCG1‐ and calcineur in‐dependent signalling pathway. Expand


Molecular Cloning: A Laboratory Manual
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Expand
Cell Tissue Kinet
  • 1984