The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro.

@article{Casper2005TheCD,
  title={The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro.},
  author={John M Casper and Michael G Kemp and Maloy Ghosh and Gia M Randall and Andrew Vaillant and M. Leffak},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 13},
  pages={13071-83}
}
The presence of DNA-unwinding elements (DUEs) at eukaryotic replicators has raised the question of whether these elements contribute to origin activity by their intrinsic helical instability, as protein-binding sites, or both. We used the human c-myc DUE as bait in a yeast one-hybrid screen and identified a DUE-binding protein, designated DUE-B, with a predicted mass of 23.4 kDa. Based on homology to yeast proteins, DUE-B was previously classified as an aminoacyl-tRNA synthetase; however, the… CONTINUE READING