The burden borne by urease.

  title={The burden borne by urease.},
  author={Brian P Callahan and Yang Yuan and Richard Wolfenden},
  journal={Journal of the American Chemical Society},
  volume={127 31},
At the active site of urease, urea undergoes nucleophilic attack by water, whereas urea decomposes in solution by elimination of ammonia so that its rate of spontaneous hydrolysis is unknown. Quantum mechanical simulations have been interpreted as indicating that urea hydrolysis is extremely slow, compared with other biological reactions proceeding spontaneously, and that urease surpasses all other enzymes in its power to enhance the rate of a reaction. We tested that possibility experimentally… CONTINUE READING

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