The broad-host-range plasmid pTF-FC2 requires a primase-like protein for autonomous replication in Escherichia coli.

@article{Dorrington1991TheBP,
  title={The broad-host-range plasmid pTF-FC2 requires a primase-like protein for autonomous replication in Escherichia coli.},
  author={Rosemary A Dorrington and Soraya Bardien and Douglas Eric Rawlings},
  journal={Gene},
  year={1991},
  volume={108 1},
  pages={7-14}
}
A 3202-bp fragment of plasmid pTF-FC2, cloned into PUC19, had previously been identified as the minimum region required for replication in either Pseudomonas aeruginosa or Escherichia coli polA- mutants. During the course of experiments to construct broad-host-range cloning vectors based on the pTF-FC2 replicon, it was found that the 3202-bp fragment had an absolute requirement for some function of the pUC19 vector. This requirement was eliminated in the presence of co-resident pTF-FC2… CONTINUE READING