The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives.

@article{Snchez2002TheBG,
  title={The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives.},
  author={C{\'e}sar S{\'a}nchez and Igor A. Butovich and Alfredo F Bra{\~n}a and J{\"u}rgen Rohr and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Chemistry \& biology},
  year={2002},
  volume={9 4},
  pages={
          519-31
        }
}
Rebeccamycin, a halogenated natural product of the indolocarbazole family, is produced by Saccharothrix aerocolonigenes ATCC39243. Several rebeccamycin analogues, which target DNA topoisomerase I or II, have already entered clinical trials as anticancer drugs. Using as a probe an internal fragment of ngt, a Saccharothrix aerocolonigenes gene encoding an indolocarbazole N-glycosyltransferase, we isolated a DNA region that directed the biosynthesis of rebeccamycin when introduced into… Expand
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