The biosynthesis in vitro of chondroitin sulphate in neonatal rat epiphysial cartilage.

@article{Handley1972TheBI,
  title={The biosynthesis in vitro of chondroitin sulphate in neonatal rat epiphysial cartilage.},
  author={Christopher J. Handley and C F Phelps},
  journal={The Biochemical journal},
  year={1972},
  volume={126 2},
  pages={417-32}
}
1. A system is described, which was used to incubate neonatal rat epiphysial cartilage in vitro with [U-(14)C]glucose and [(35)S]sulphate. 2. The acid glycosaminoglycans of neonatal rat epiphyses were extracted and fractionated on cetylpyridinium chloride-cellulose columns. The major components were chondroitin 4-sulphate (65%), chondroitin 6-sulphate (15%), hyaluronic acid (4%) and keratan sulphate (2%). 3. The acid-soluble nucleotides and intermediates of glycosaminoglycan synthesis were… CONTINUE READING

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