The biochemistry and fidelity of synthesis by the apicoplast genome replication DNA polymerase Pfprex from the malaria parasite Plasmodium falciparum.

@article{Kennedy2011TheBA,
  title={The biochemistry and fidelity of synthesis by the apicoplast genome replication DNA polymerase Pfprex from the malaria parasite Plasmodium falciparum.},
  author={Scott R Kennedy and Cheng-Yao Chen and Michael W. Schmitt and Cole N Bower and L. A. Loeb},
  journal={Journal of molecular biology},
  year={2011},
  volume={410 1},
  pages={27-38}
}
Plasmodium falciparum, the major causative agent of human malaria, contains three separate genomes. The apicoplast (an intracellular organelle) contains an ∼35-kb circular DNA genome of unusually high A/T content (>86%) that is replicated by the nuclear-encoded replication complex Pfprex. Herein, we have expressed and purified the DNA polymerase domain of Pfprex [KPom1 (Klenow-like polymerase of malaria 1)] and measured its fidelity using a LacZ-based forward mutation assay. In addition, we… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 34 references

Similar Papers

Loading similar papers…