The biochemical effects of extracellular Zn(2+) and other metal ions are severely affected by their speciation in cell culture media.

@article{Haase2015TheBE,
  title={The biochemical effects of extracellular Zn(2+) and other metal ions are severely affected by their speciation in cell culture media.},
  author={Hajo Haase and Silke Hebel and Gabriela Engelhardt and Lothar Rink},
  journal={Metallomics : integrated biometal science},
  year={2015},
  volume={7 1},
  pages={102-11}
}
Investigations of physiological and toxicological effects of metal ions are frequently based on in vitro cell culture systems, in which cells are incubated with these ions in specialized culture media, instead of their physiological environment. This allows for targeted examination on the cellular or even molecular level. However, it disregards one important aspect, the different metal ion speciation under these conditions. This study explores the role of culture conditions in investigations… CONTINUE READING