The binding of in vitro synthesized adenovirus DNA binding protein to single-stranded DNA is stimulated by zinc ions.

Abstract

We have synthesized wild type DNA binding protein (DBP) of adenovirus type 5 (Ad5) and several truncated forms of this protein by a combination of in vitro transcription and translation. The proteins obtained were tested for binding to a single-stranded DNA-cellulose column. It could be shown that for binding of in vitro synthesized wild type DBP, it is… (More)

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