The binding of fucose-containing glycoproteins by hepatic lectins. The binding specificity of the rat liver fucose lectin.

@article{Lehrman1986TheBO,
  title={The binding of fucose-containing glycoproteins by hepatic lectins. The binding specificity of the rat liver fucose lectin.},
  author={Mark A. Lehrman and Robert S. Haltiwanger and Robert L. Hill},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 16},
  pages={7426-32}
}
The parameters that affect the interaction of ligands with a fucose-binding lectin from rat liver have been examined. 125I-Fucosyl-bovine serum albumin (Fuc-BSA) containing 50 residues of fucose/molecule was used as the standard ligand. At low initial concentrations of ligand (10 ng/ml) and lectin (140 ng/ml), the reaction reaches equilibrium at pH 7.8, 23 degrees C, within 40 min. The binding of ligands is Ca2+ dependent with half-maximal binding occurring at 54 microM Ca2+; of several metal… CONTINUE READING

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