The binding of drugs to hepatocytes and its relationship to physicochemical properties.

@article{Austin2005TheBO,
  title={The binding of drugs to hepatocytes and its relationship to physicochemical properties.},
  author={Rupert P. Austin and Patrick Barton and Sarfraz Mohmed and Rob Riley},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2005},
  volume={33 3},
  pages={419-25}
}
The binding of 17 drugs to rat hepatocytes has been determined using equilibrium dialysis in combination with metabolic inhibitors and a kinetic model for the binding and dialysis processes. Metabolic inhibitors were used to retard the main routes of metabolism such that the half-life for turnover of the drugs was comparable to or greater than the time scale of the equilibrium dialysis process. Further experiments were carried out to determine the kinetics of diffusion of the compounds across… CONTINUE READING