The binding of antibiotics to serum proteins.

@article{Rolinson1965TheBO,
  title={The binding of antibiotics to serum proteins.},
  author={George Newbolt Rolinson and Robert Sutherland},
  journal={British journal of pharmacology and chemotherapy},
  year={1965},
  volume={25 3},
  pages={
          638-50
        }
}
ultrafiltration Visking dialysis tubing. Preliminary experiments indicated that there was no significant difference between the extent of binding in plasma obtained from heparinized blood and that in serum. The antibiotic present in the protein-free ultrafiltrate was measured by microbiological assay and this quantity represented the free, unbound, fraction of antibiotic in serum. The amount of antibiotic bound to protein was derived by subtracting the level of free antibiotic from the known… 

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