The beta subunit of tryptophan synthase. Clarification of the roles of histidine 86, lysine 87, arginine 148, cysteine 170, and cysteine 230.

@article{Miles1989TheBS,
  title={The beta subunit of tryptophan synthase. Clarification of the roles of histidine 86, lysine 87, arginine 148, cysteine 170, and cysteine 230.},
  author={Edith Wilson Miles and Haruhiko Kawasaki and S. Ashraf Ahmed and Hiroyuki Morita and Shinji Nagata},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 11},
  pages={6280-7}
}
Our studies, which are aimed at understanding the catalytic mechanism of the beta subunit of tryptophan synthase from Salmonella typhimurium, use site-directed mutagenesis to clarify the functional roles of several putative active site residues. Although previous chemical modification studies have suggested that histidine 86, arginine 148, and cysteine 230 are essential residues in the beta subunit, our present findings that beta subunits with single amino acid replacements at these positions… CONTINUE READING