The bceT gene of Bacillus cereus encodes an enterotoxic protein.

  title={The bceT gene of Bacillus cereus encodes an enterotoxic protein.},
  author={Norio Agata and Mika Ohta and Yoshichika Arakawa and Masataka Mori},
  volume={141 ( Pt 4)},
A toxin gene (bceT) on a 2.9 kb DNA fragment of Bacillus cereus B-4ac was cloned and expressed in Escherichia coli, and its nucleotide sequence determined. The DNA fragment contained an open reading frame capable of encoding a polypeptide of 336 amino acids with a molecular mass of 41039 Da. The translated product in E. coli exhibited Vero cell cytotoxicity, and was positive in a vascular permeability assay. It also caused fluid accumulation in a ligated mouse ileal loop and was lethal to mice… CONTINUE READING


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