The bceT gene of Bacillus cereus encodes an enterotoxic protein.

@article{Agata1995TheBG,
  title={The bceT gene of Bacillus cereus encodes an enterotoxic protein.},
  author={Norio Agata and Mika Ohta and Yoshichika Arakawa and Masataka Mori},
  journal={Microbiology},
  year={1995},
  volume={141 ( Pt 4)},
  pages={983-8}
}
A toxin gene (bceT) on a 2.9 kb DNA fragment of Bacillus cereus B-4ac was cloned and expressed in Escherichia coli, and its nucleotide sequence determined. The DNA fragment contained an open reading frame capable of encoding a polypeptide of 336 amino acids with a molecular mass of 41039 Da. The translated product in E. coli exhibited Vero cell cytotoxicity, and was positive in a vascular permeability assay. It also caused fluid accumulation in a ligated mouse ileal loop and was lethal to mice… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 48 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 23 references

A Short Course in Bacterial Genetics

J. H. Miller
Cold Spring Harbor, • 1992
View 4 Excerpts
Highly Influenced

Studies on the production of enterotoxins by Bacillus cereus.

Journal of clinical pathology • 1976
View 6 Excerpts
Highly Influenced

Bacillus cereus and other Bacillus species

Kramer, M 1., R. J. Gilbert
In Foodborne Bacterial Pathogens, • 1988

A new strategy for ordered DNA sequencing based on a novel method for the rapid purification of near-milligram quantities of a cloned restriction fragment

M. S. Gilmore, Gilmore, W. Goebel
Gene Anal Tech • 1985

Similar Papers

Loading similar papers…