The base specificities of tomato ribonuclease (RNase LE) and its Asp44 mutant enzyme expressed from yeast cells.

@article{Ohgi1997TheBS,
  title={The base specificities of tomato ribonuclease (RNase LE) and its Asp44 mutant enzyme expressed from yeast cells.},
  author={Kazuko Ohgi and Yasuhiko Shiratori and Akihito Nakajima and M. Iwama and Hiroko Kobayashi and Norio Inokuchi and Takashi Koyama and Matthias K{\"o}ck and Andreas L{\"o}ffler and Konrad Glund and Masachika Irie},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1997},
  volume={61 3},
  pages={432-8}
}
RNase LE from cultured tomato cells is a member of the RNase T2 family. It is, however, distinguishable from RNase Rh from Rhizopus niveus, a typical RNase of the RNase T2 family, by its CD spectrum in the 200-250 nm region. In order to reinvestigate the base specificity of RNase LE and to study the role of Asn44 in RNase LE, which is considered to correspond to the base recognition site Asp51 of RNase Rh, RNase LE, and its Asp mutant at the 44th position were expressed from yeast cells with… CONTINUE READING