The bacteriophage T4 insertion/substitution vector system. A method for introducing site-specific mutations into the virus chromosome.

@article{Selick1988TheBT,
  title={The bacteriophage T4 insertion/substitution vector system. A method for introducing site-specific mutations into the virus chromosome.},
  author={H E Selick and K. Kreuzer and Bruce M. Alberts},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 23},
  pages={11336-47}
}
A bacteriophage T4 insertion/substitution vector system has been developed as a means of introducing in vitro generated mutations into the T4 chromosome. The insertion/substitution vector is a 2638-base pair plasmid containing the pBR322 origin of replication and ampicillin resistance determinant, a T4 gene 23 promoter/synthetic supF tRNA gene fusion, and a polylinker with eight unique restriction enzyme recognition sites. A T4 chromosomal "target" DNA sequence is cloned into this vector and… CONTINUE READING