The azurin gene from Pseudomonas aeruginosa. Cloning and characterization.

We have cloned and sequenced the Pseudomonas aeruginosa azurin structural gene and its flanking regions. The DNA sequence predicts a pre-protein with a signal peptide of 19 amino acids followed by the 128-amino-acid mature azurin protein. Nuclease-S1 mapping and primer elongation experiments indicated two 5' termini of the azurin transcript. The major… CONTINUE READING