The autophagosome: origins unknown, biogenesis complex

@article{Lamb2013TheAO,
  title={The autophagosome: origins unknown, biogenesis complex},
  author={Christopher A. Lamb and Tamotsu Yoshimori and Sharon A. Tooze},
  journal={Nature Reviews Molecular Cell Biology},
  year={2013},
  volume={14},
  pages={759-774}
}
Healthy cells use autophagy as a general 'housekeeping' mechanism and to survive stress, including stress induced by nutrient deprivation. Autophagy is initiated at the isolation membrane (originally termed the phagophore), and the coordinated action of ATG (autophagy-related) proteins results in the expansion of this membrane to form the autophagosome. Although the biogenesis of the isolation membrane and the autophagosome is complex and incompletely understood, insight has been gained into… 
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References

SHOWING 1-10 OF 154 REFERENCES
The role of Atg proteins in autophagosome formation.
TLDR
The molecular mechanism of autophagosome formation is described with particular focus on the function of Atg proteins and the long-standing discussion regarding the origin of the autophagous membrane membrane.
The molecular machinery of autophagy: unanswered questions
  • D. Klionsky
  • Biology, Chemistry
    Journal of Cell Science
  • 2005
Autophagy is a process in which cytosol and organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation and recycling of the resulting
Endocytosis and autophagy: Shared machinery for degradation
TLDR
Recent findings highlighting the role of the classical endosomal pathway, from plasma membrane to lysosome, in the formation and expansion of the phagophore and subsequent degradation of the autophagosome contents are discussed.
A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
TLDR
Electron tomography revealed that the ER–IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.
Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
TLDR
The results indicate that the autophagic and secretory pathways intersect at a level preceding the brefeldin A blockage, suggesting that the transport from the cis/medial Golgi is not necessary for autophagosome biogenesis.
Antibacterial autophagy occurs at PI(3)P-enriched domains of the endoplasmic reticulum and requires Rab1 GTPase
TLDR
The data suggest that antibacterial autophagy occurs at omegasomes and reveal that the Rab1 GTPase plays a crucial role in mammalian Autophagy.
The LIR motif – crucial for selective autophagy
TLDR
Comment on new insights on the interactions of LIR-containing proteins with members of the ATG8 protein family on the interaction of autophagy receptors to LC3-interacting region proteins anchored in the phagophore membrane.
Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae
TLDR
This study visualized expanding IMs as cup-shaped structures using fluorescence microscopy by enlarging a selective cargo of autophagosomes, and finely mapped the localizations of Atg proteins, suggesting that AtG proteins play individual roles at spatially distinct locations during IM expansion.
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