The auto-orientation in high magnetic fields of oxidized cytochrome b562 as source of constraints for solution structure determination

@article{Arnesano2000TheAI,
  title={The auto-orientation in high magnetic fields of oxidized cytochrome b562 as source of constraints for solution structure determination},
  author={Fabio Arnesano and Lucia Banci and Ivano Bertini and Karin van der Wetering and Michael Czisch and R. Kaptein},
  journal={Journal of Biomolecular NMR},
  year={2000},
  volume={17},
  pages={295-304}
}
Abstract15N-1H 1J couplings were measured at 500 MHz and 800 MHz for 15N enriched oxidized cytochrome b562 from E. coli. The magnetic field dependence of 70 1J values, which could be measured without signal overlap, shows that there is a molecular magnetic anisotropy which provides partial molecular orientation in the magnetic field and, consequently, residual dipolar couplings (rdc). The rdc were used as further constraints to improve the existing structure [Arnesano et al. (1999) Biochemistry… 
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References

The solution structure of paramagnetic metalloproteins.