The association of thymidine kinase activity and thymidine transport in Escherichia coli.

@article{Dube1991TheAO,
  title={The association of thymidine kinase activity and thymidine transport in Escherichia coli.},
  author={Dipak K Dube and Marshall S Horwitz and L. A. Loeb},
  journal={Gene},
  year={1991},
  volume={99 1},
  pages={25-9}
}
We have constructed a series of mutants within the putative nucleoside-binding site of the herpes simplex type-1 virus (HSV-1) thymidine kinase (TK)-encoding gene (tk), contained within an expression vector. While most mutations within this sequence produce an inactive protein, we find no absolute requirement for the wild-type Ile166 and Ala167. The uptake of thymidine (dT) into Escherichia coli tdk-, lacking functional endogenous TK activity, is proportional to the amount of TK activity… CONTINUE READING

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